Abstract: Ultrasound-guided abdominal lipoaspiration is a minimally invasive method for harvesting autologous adipose-derived stem cells for orthopaedic regenerative applications. This outpatient procedure ensures precise tissue harvesting to obtain high-quality injectable microfragmented adipose tissue. This Technical Note outlines a standardized protocol for ultrasound-guided abdominal lipoaspiration and adipose tissue processing. We emphasize the benefits of ultrasound use in optimizing cell yield and minimizing procedural risks.

By: Balaji Sridhar, M.D., Ph.D., M.P.H., Michelle Katz, B.Sc., Nishit Pancholi, M.D., M.Sc., Nathan Katz, Ph.D., Ryan Dregalla, Ph.D., and Ariana DeMers, D.O., R.M.S.K.

View of hypoechogenic fluid in deep layer of fat - Ultrasound-guided abdominal lipoaspiration.
View of hypoechogenic fluid in deep layer of fat after injection with tumescent solution. The blue arrow is pointing at the cannula tip.

You can review this advanced, practical method in Arthroscopy Techniques.

Full text is available at: https://www.arthroscopytechniques.org/article/S2212-6287(25)00225-7/fulltext